![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2787012:Design
Designed by: Haoer Shi Group: iGEM18_ShanghaiTech (2018-10-09)
GFP under A2 orthogonal RBS with orthogonal 16S rRNA BBa_K2787040
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 797
Illegal NheI site found at 820 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1660
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1674
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706
Design Notes
Two parts were cloned into one plasmid to avoid issues brought by co-transformation.
Source
The part is constructed by restriction cloning BBa_K2787008 and BBa_K2787000 together into pSB1C3 backbone.